产品线涵盖分子生物、细胞培养、样品处理、微生物学、样品冻存、免疫检测、核酸提取、蛋白纯化、食品检测等方面,为客户提供品质产品及专业技术服务。
Imag磁珠分选、凋亡、增殖与周期、BD CBA多重蛋白定量技术、T细胞研究、ELISA/ELISPOT、BD Phosflow磷酸化蛋白流式分析、WB/IHC/IP/IF/Bioimg、干细胞完整解决方案
Imag磁珠分选、凋亡、增殖与周期、BD CBA多重蛋白定量技术、T细胞研究、ELISA/ELISPOT、BD Phosflow磷酸化蛋白流式分析、WB/IHC/IP/IF/Bioimg、干细胞完整解决方案
碧迪生物科学BD Pharmingen 试剂精选
BD Pharmingen 产品线主要提供流式相关试剂,包括流式抗体,细胞功能检测试剂,死活染料,CBA流式液相多重蛋白定量检测试剂盒,磷酸化抗体以及诸多流式辅助试剂。
Imag磁珠分选、凋亡、增殖与周期、BD CBA多重蛋白定量技术、T细胞研究、ELISA/ELISPOT、BD Phosflow磷酸化蛋白流式分析、WB/IHC/IP/IF/Bioimg、干细胞完整解决方案
1、机器日程维护和质控很重要,一定要记得使用质控微球
产品编号 | 产品名称 | 规格 | 用途 |
340486 | BD Calibrite™ 3 Beads | 25T | FACSCalibur 3色质控微球 |
340487 | BD Calibrite™ APC Beads | 25T | FACSCalibur APC质控微球 |
335775 | BD FACS™ 7-Color Setup Beads | 25T | FACSCanto 7色设置微球 |
345249 | BD FACS™ Accudrop Beads | 25T | 分选仪Accdrop微球 |
650621 | BD FACSuite™ CS&T Research Beads | 50T | FACSVerse质控微球 |
650622 | BD FACSuite™ CS&T Research Beads | 150T | FACSVerse质控微球 |
655050 | BD FACSDiva™ CS&T Research Beads | 50T | Diva质控(Diva7及以上) |
655051 | BD FACSDiva™ CS&T Research Beads | 150T | Diva质控(Diva7及以上) |
641319 | BD™ Cytometer Setup & Tracking Beads Kit | 50T | Diva质控(Diva6.X) |
642412 | BD™ Cytometer Setup & Tracking Beads Kit | 150T | Diva质控(Diva6.X) |
653144 | BD Accuri Spherotech 8-Peak Beads(FL1-FL3) | 4ml | Accuri质控微球 |
653145 | BD Accuri Spherotech 6-Peak Beads(FL4) | 8ml | Accuri质控微球 |
556291 | Sphero™ Rainbow Fluorescent Particles, 3.0 - 3.4 µm | 5ml | Rainbow质控微球 |
342003 | BD FACSFlow™ Sheath Fluid | 20L | 鞘液 |
340345 | BD FACS™ BD FACS Clean Solution | 5L | 清洗液 |
334224 | BD FACS™ BD FACS Shutdown Solution | 5L | FACSCanto关机液 |
340334 | BD Trucount™ Absolute Counting Tubes | 50T | 绝对计数管 |
653160 | BD Accuri™ C6 Flow Cytometer Starter Kit | Kit | Accuri启动套装 |
653158 | BD Accuri™ C6 Flow Cytometer Fluid Kit | Kit | Accuri清洗液套装 |
653149 | BD Accuri™ C6 Flow Cytometer 1-year Maintenance Kit | Kit | Accuri保养套装(一年) |
653150 | BD Accuri™ C6 Flow Cytometer 2-month Maintenance Kit | Kit | Accuri保养套装(2个月) |
更多.... |
2、样本制备推荐产品:红细胞裂解液和滤网
货号 | 产品名称 | 规格 |
555899 | Lyse™ Lysing Buffer(10X) | 100ml |
352340 | 细胞滤网40μm/蓝色 | 50个/箱 |
352350 | 细胞滤网70μm/白色 | 50个/箱 |
352360 | 细胞滤网100μm/黄色 | 50个/箱 |
更多.... |
3、FC受体阻断剂:避免FC非特异性反应
Fc受体是指细胞表面能够与免疫球蛋白(IgG、IgA、IgM、IgE和IgD)集合的分子。其广泛存在于NK细胞、肥大细胞、巨噬细胞、中性粒细胞的表面。FcR能够与抗体的Fc段结合,在检测时产生假阳性。使用FcR阻断剂,可以消除假阳性,降低检测背景,获得更清晰的结果。
产品名称 | 产品形式 | 规格 | 产品编号 |
Human BD FC Block | Purified | 50ug | 564219 |
0.25mg | 564420 | ||
Mouse BD FC Block | Purified | 0.1mg | 553141 |
0.5mg | 553142 | ||
Rat BD FC Block | Purified | 0.1mg | 550270 |
0.5mg | 550271 |
4、凋亡试剂盒/周期/增殖
产品编号 | 产品名称 | 规格 | 用途 |
556547 | FITC Annexin V Apoptosis Detection Kit I | 100T | 凋亡试剂盒(Annexin-FITC,PI) |
559763 | PE Annexin V Apoptosis Detection Kit I | 100T | 凋亡试剂盒(Annexin-PE,7-AAD) |
550825 | PI/RNase Staining Buffer | 100ml | 细胞周期检测 |
559619 | FITC Brd U Flow Kit | 50tests | Brdu增殖试剂盒 |
更多... |
5、新机器要用起来,准备开始流式实验!选抗体,BD,BD,还是BD.. ! 可以按照如下搭配好的panel直接选择
常见human Panel搭配
亚 群 | 货 号 | 描 述 | 规 格 |
T 细胞 | 564420 | HU CD4 BB515 | 25T |
561949 | HU CD8 PE | 25T | |
560835 | HU CD3 PERCP-CY5.5 | 50T | |
T 细胞 | 560835 | HU CD3 PERCP-CY5.5 | 50T |
564419 | Hu CD4 BB515 | 100T | |
555367 | HU CD8 PE | 100T | |
B 细胞 | 561806 | HU CD3 FITC | 25T |
561742 | HU CD19 APC | 25T | |
B 细胞 | 555332 | HU CD3 FITC | 100T |
555415 | HU CD19 APC | 100T | |
NK 细胞 | 561806 | HU CD3 FITC | 25T |
561724 | HU CD16 ALEXA 647 | 25T | |
561903 | HU CD56 PE | 25T | |
NK 细胞 | 555332 | HU CD3 FITC | 100T |
557710 | HU CD16 ALEXA 647 | 100T | |
555516 | HU CD56 PE | 100T | |
DC | 552764 | HU HLA-DR PERCP-CY5.5 | 50T |
560895 | HU CD11C APC | 25T | |
561050 | HU CD123 PE | 25T | |
565009 | HU CD80 BB515 | 25T | |
560957 | HU CD86 PE | 25T | |
561960 | HU CD83 APC | 25T | |
DC | 552764 | HU HLA-DR PERCP-CY5.5 | 50T |
559877 | HU CD11C APC | 100T | |
555644 | HU CD123 PE | 200T | |
565008 | HU CD80 BB515 | 100T | |
555658 | HU CD86 PE | 100T | |
551073 | HU CD83 APC | 100T | |
单核细胞 | 561712 | HU CD14 FITC | 25T |
单核细胞 | 555397 | HU CD14 FITC | 100T |
Treg | 564420 | HU CD4 BB515 | 25T |
560987 | HU CD25 APC | 25T | |
560082 | HU FOXP3 PE | 25T | |
562725 | TRANSCRIPTION FACTOR BUFFER SET 25T | 25T | |
Treg | 564419 | HU CD4 BB515 | 100T |
555434 | HU CD25 APC | 100T | |
560046 | HU FOXP3 PE | 100T | |
562574 | TRANSCRIPTION FACTOR BUFFER SET 100T | 100T | |
Treg | 564420 | HU CD4 BB515 | 25T |
560987 | HU CD25 APC | 25T | |
561028 | HU CD127 PE | 25T | |
Treg | 564419 | HU CD4 BB515 | 100T |
555434 | HU CD25 APC | 100T | |
557938 | HU CD127 PE | 100T | |
TH1/TH2/TH17 | 564420 | HU CD4 BB515 | 25T |
560704 | HU IFN-GAMMA NHP PERCP-CY5.5 | 50T | |
560671 | HU IL-4 APC | 50T | |
560438 | HU IL-17A PE | 25T | |
554714 | CYTOFIX/CYTOPERM BUF KIT | 250T | |
550583 | LEUKO ACTVTN CKTL WITH GLGPLG 100UL X2 | 200ìL | |
TH1/TH2/TH17 | 564419 | HU CD4 BB515 | 100T |
560704 | HU IFN-GAMMA NHP PERCP-CY5.5 | 50T | |
560671 | HU IL-4 APC | 50T | |
560436 | HU IL-17A PE | 100T | |
554714 | CYTOFIX/CYTOPERM BUF KIT | 250T | |
550583 | LEUKO ACTVTN CKTL WITH GLGPLG 100UL X2 | 200ìL | |
Tfh | 564419 | HU CD4 BB515 | 100T |
562781 | HU CXCR5 PERCP-CY5.5 | 50T | |
558694 | HU CD279 APC | 100T | |
557802 | HU CD278 PE | 100T | |
肿瘤干细胞 | 560991 | HU CD24 PE | 25T |
560977 | HU CD44 FITC | 25T | |
564105 | HU CD45 PERCP-CY5.5 | 100T | |
肿瘤干细胞 | 555428 | HU CD24 PE | 100T |
555478 | HU CD44 FITC | 100T | |
564105 | HU CD45 PERCP-CY5.5 | 100T | |
白细胞 | 564106 | HU CD45 PERCP-CY5.5 | 25T |
造血干细胞 | 555822 | HU CD34 PE | 100T |
红细胞 | 561901 | HU CD55 PE | 25T |
560954 | HU CD59 FITC | 25T | |
561051 | HU CD235A PE | 25T | |
红细胞 | 555694 | HU CD55 PE | 100T |
555763 | HU CD59 FITC | 100T | |
555570 | HU CD235A PE | 100T | |
血小板 | 561913 | HU CD61 FITC | 25T |
561921 | HU CD62P PE | 25T | |
血小板 | 555753 | HU CD61 FITC | 100T |
555524 | HU CD62P PE | 100T | |
粒细胞 | 561698 | HU CD13 APC | 25T |
561818 | HU CD33 FITC | 25T | |
粒细胞 | 557454 | HU CD13 APC | 100T |
555626 | HU CD33 FITC | 100T |
6、常用mouse Panel搭配
亚 群 | 货 号 | 描 述 | 规 格 |
T细胞 | 561835 | MS CD4 FITC | 25T |
561095 | MS CD8A PE | 25T | |
561108 | MS CD3E PERCP-CY5.5 | 25T | |
T细胞 | 553046 | MS CD4 FITC | 100T |
553032 | MS CD8A PE | 100T | |
551163 | MS CD3E PERCP-CY5.5 | 100T | |
B细胞 | 561738 | MS CD19 APC | 25T |
561878 | MS CD45R PE | 25T | |
B细胞 | 550992 | MS CD19 APC | 100T |
553089 | MS CD45R PE | 100T | |
NK 细胞 | 557391 | MS NK-1.1 PE | 100T |
558759 | MS CD49B PE | 100T | |
DC树突细胞 | 562363 | MS I-A I-E PERCP-CY5.5 | 50T |
550261 | MS CD11C APC | 100T | |
561954 | MS CD 80 FITC | 100T | |
561963 | MS CD86 PE | 25T | |
558206 | MS CD83 APC | 100T | |
单核细胞 | 557396 | MS CD11B FITC | 100T |
粒细胞 | 553126 | MS LY-6G LY-6C FITC | 100T |
565410 | MS F4/80 PE | 100T | |
Treg细胞 | 561835 | MS CD4 FITC | 25T |
561048 | MS CD25 APC | 25T | |
563101 | MS FOXP3 PE | 100T | |
562725 | TRANSCRIPTION FACTOR BUFFER SET 25T | 25T | |
Treg细胞 | 553046 | MS CD4 FITC | 100T |
557192 | MS CD25 APC | 100T | |
563101 | MS FOXP3 PE | 100T | |
562574 | TRANSCRIPTION FACTOR BUFFER SET | 100T | |
TH1/TH2/TH17 | 561835 | MS CD4 FITC | 25T |
560660 | MS IFN-GAMMA PERCP-CY5.5 5 | 50T | |
562045 | MS IL-4 APC | 25T | |
561020 | MS IL-7A PE | 25T | |
554714 | CYTOFIX/CYTOPERM BUF KIT | 250T | |
550583 | LEUKO ACTVTN CKTL WITH GLGPLG 100UL X2 | 200ul | |
TH1/TH2/TH17 | 553046 | MS CD4 FITC | 100T |
560660 | MS IFN-GAMMA PERCP-CY5.5 | 50T | |
554436 | MS IL-4 APC | 100T | |
559502 | MS IL-17A PE | 100T | |
554714 | CYTOFIX/CYTOPERM BUF KIT | 250T | |
550583 | LEUKO ACTVTN CKTL WITH GLGPLG 100UL X2 | 200ul | |
其他常见搭配/辅助试剂
种属 | 亚群 | 货 号 | 描 述 | 规 格 |
rat | Rat T Lymphocyte Cocktail | 558493 | RAT T LYM CKTL | 50T |
Isotype Control Cocktail B | 558508 | ISOT CTL CKTL B | 20T | |
Rat Activated T Lymphocyte Cocktail | 558494 | RAT T LYM ACT CKTL | 50T | |
Rat T/B/NK Cell Cocktail | 558495 | RAT T/B/NK CELL CKTL | 50T | |
dog | Dog T Lymphocyte Cocktail | 558699 | DOG T LYMPHOCYTE COCKTAIL FITC,PE, APC | 50T |
Dog Activated T Lymphocyte Cocktail | 558704 | DOG ACT CKTL | 50T | |
pig | T 细胞 | 559582 | PIG CD3 EPSL FITC | 100T |
561474 | PIG CD4A PERCP-CY5.5 | 50T | ||
559584 | PIG CD8A PE | 100T | ||
Isotype control | 556649 | FITC Mouse IgG1, ê Isotype Control | 50T | |
552834 | PerCP-Cy5.5 Mouse IgG1 ê Isotype Control | 50T | ||
550854 | APC Mouse IgG1 ê Isotype Control | 50T | ||
556652 | FITC Mouse IgG2a, ê Isotype Control | 50T | ||
556655 | FITC Mouse IgG2b, ê Isotype Control | 50T | ||
552916 | FITC Rat IgG1 ê Isotype Control | 50T | ||
551979 | PE Rat IgG1, ê Isotype Control | 50T | ||
560537 | PerCP-Cy5.5 Rat IgG1, ê Isotype Control | 0.1MG | ||
554686 | APC Rat IgG1, ê Isotype Control | 0.1MG | ||
NA/LE抗体 | 555336 | Purified NA/LE Mouse Anti-Human CD3 HIT3a | 0.5MG | |
555725 | Purified NA/LE Mouse Anti-Human CD28 CD28.2 | 0.5MG | ||
553057 | Purified NA/LE Hamster Anti-Mouse CD3e 145-2C11 | 0.5MG | ||
553294 | Purified NA/LE Hamster Anti-Mouse CD28 37.51 | 0.5MG |
7、细胞因子检测看过来,BD CBA多因子检测方法省钱、省时间、省样本—50ul样本最多检测30多种细胞因子,有了流式细胞仪,方便!
CBA经典产品
产品名称 | 产品编号 | 检测指标 | 包装 |
Human Inflammatory Cytokine Kit | 551811 | IL-8, IL-1β, IL-6, IL-10, TNF, IL-12p70 | 80T |
Human Th1/Th2 Cytokine Kit II | 551809 | IL-2, IL-4, IL-6, IL-10, TNF, IFN-g | 80T |
Human Th1/Th2/Th17 Kit | 560484 | IL-2, IL-4, IL-6, IL-10, TNF, IFN-γ, IL-17A | 80T |
Mouse Inflammation Kit | 552364 | L-6, IL-10, MCP-1, IFN-γ, TNF, IL-12p70 | 80T |
Mouse Th1/Th2/Th17 Cytokine Kit | 560485 | IL-2, IL-4, IL-6, IFN-γ, TNF, IL-17A, IL-10 | 80T |
Mouse Th1/Th2 Cytokine Kit | 551287 | IL-2, IL-4, IL-5, IFN-γ,TNF | 80T |
Non-human Primate Th 1 , /Th 2 Cytokine Kit | 557800 | IL-2, IL-4, IL-5, IL-6,TNF, IFN-γ | 80T |
更多..... |
8、磁珠分选和分选型仪器仪器使用更配哦,超高性价比!
磁珠分选经典产品:
目的细胞 | 产品编号 | 产品名称 | 规格 |
Human T细胞 | 557874 | Hu T Lymphocyte Enrichment Set - DM | set |
Human CD4+ T细胞 | 557939 | Hu CD4 T Lymphocyte Enrichment Set - DM | set |
Human CD8+T细胞 | 557941 | Hu CD8 T Lymphocyte Enrichment Set - DM | set |
Human B细胞 | 558007 | Hu B Lymphocyte Enrichment Set -DM | set |
Human NK细胞 | 557987 | Hu NK Cell Enrichment Set - DM | set |
Mouse T细胞 | 557793 | Ms T Lymphocyte Enrichment Set -DM | set |
Mouse CD4+T细胞 | 558131 | Ms CD4 T Lymphocyte Enrichment Set - DM | set |
Mouse CD8+T细胞 | 558471 | Ms CD8 T Lymphocyte Enrichment Set - DM | set |
Mouse B细胞 | 557792 | Ms B Lymphocyte Enrichment Set -DM | set |
Mouse NK细胞 | 557954 | Ms NK Cell Enrichment Set - DM | set |
Mouse DC树突细胞 | 557955 | Ms Dendritic Cell Enrichment Set - DM | set |
PE间标磁珠 | 557899 | Anti-PE Magnetic Particles - DM E31-1459 | set |
APC间标磁珠 | 557932 | APC Magnetic Particles - DM | set |
链酶亲和素间标磁珠 | 557812 | Streptavidin Particles Plus - DM | set |
磁珠分选用buffer | 552362 | Buffer (10x) for use with BD IMag ™cell separation products | set |
9、补偿微球
由于荧光光谱的重叠现象,在进行流式多色分析时,必须设置单染管进行荧光补偿。OneComp/UltraComp eBeads微球大小和淋巴细胞相当,可连接各种荧光标记的抗体来调节补偿。每滴微球中都含有两群:一群是可以结合抗体的阳性群,一群是不会与抗体反应的阴性群。
优点:
•使用方便,一滴即可
•与所有小鼠、大鼠和仓鼠来源的抗体反应,要求抗体来源是Kappa
•适用于各种激发光—适用于488nm蓝光,532nm绿光,561nm黄光,633-635nm红光,UltrComp eBeads尤其适用紫外紫色(405nm)激光器。
1)CompBead Compensation Particles 通用补偿微球
产品编号 | 产品名称 | 规格 | 反应 | 适合激光器 |
552844 | Anti-rat Ig, k | 6ml | 适用于rat来源的一抗 | 蓝(488 nm), 绿(532 nm), 黄绿 (561 nm), |
552845 | Anti-rat/hamster Ig, k | 6ml | 适用于rat/hamster来源的一抗 | 蓝(488 nm), 绿(532 nm), 黄绿 (561 nm), |
552843 | Anti-mouse Ig, k | 6ml | 适用于mouse来源的一抗 | 蓝(488 nm), 绿(532 nm), 黄绿 (561 nm), |
2)CompBead Plus Particles 补偿大微球,适用于larger cell types, for example, human embryonic stem cells (hESCs)
产品编号 | 产品名称 | 规格 | 反应 | 适合激光器 |
560499 | Anti-rat Ig, k | 6ml | 适用于rat来源的一抗 | 蓝(488 nm), 绿(532 nm), 黄绿 (561 nm), |
560497 | Anti-mouse Ig, k | 6ml | 适用于mouse来源的一抗 | 蓝(488 nm), 绿(532 nm), 黄绿 (561 nm), |
10、细胞死活鉴定染料 Fxiable Viability Stain(FVS)Reagents
为什么要鉴定细胞死活?
• 死细胞非特异性染色使细胞背景增高,影响检测灵敏度
• 鉴定细胞生命活性
• 死细胞假阳性染色结果影响真实实验结果比例
与传统染料PI、7-AAD相比的优势?适用于胞内染色的死活细胞鉴定
①、PI、7-AAD的染色通常是放在其他抗体孵育完成后进行,不能洗涤即要上机检测;
②、对于胞内抗体的染色,由于经过了固定步骤,PI或7-AAD染色后所有的细胞都有阳性,所以它们只适合胞膜染色的死活细胞鉴定。
③、PI、7-AAD与常用染色通道PE、PercP是统一检测通道,二者只能选择其一使用。PI和7-AAD发射谱很宽,488nm蓝色激光发射基本上580BP和690BP都被占用,这样大大减小多色试验中的配色增加了调补偿的难度。
④、Fixable Viability Dye的优点是:
—固定、破膜以及洗涤步骤不影响Fixable Viability Dye的染色
—FVS系列光谱多样,适合各种染色组合的搭配。FVS系列分别对应不同的激光器,发射波长不同,波谱集中。
产品编号 | 产品名称 | 规格 | 所在荧光通道 |
562247 | Fxiable Viability Stain450 | 紫激光 | BV421/V450 |
564406 | Fxiable Viability Stain510 | BV | |
564407 | Fxiable Viability Stain520 | 蓝激光 | 适用于mouse来源的一抗 |
564995 | Fxiable Viability Stain570 | 适用于mouse来源的一抗 | |
564996 | Fxiable Viability Stain620 | 适用于mouse来源的一抗 | |
564996 | Fxiable Viability Stain620 | 黄/绿激光 | 适用于mouse来源的一抗 |
564405 | Fxiable Viability Stain660 | 红激光 | 适用于mouse来源的一抗 |
564997 | Fxiable Viability Stain700 | 适用于mouse来源的一抗 |
Fixable Viability Dye使用原理
FVS为氨基反应性染料,与细胞表面或者内部的蛋白不可逆的结合。FVS是染细胞表面或膜内侧的游离胺,活细胞弱阳。正是由于和PI、7-AAD原理不同,所以对于一些需要固定破膜步骤的试验FVS一样适用,如果固定破膜后细胞发生死亡,该染料还是能反应细胞的死活情况,这点是核酸染料所不能替代的。
周一至周五:9:00—18:00
周六、周日:9:00—16:00
福州木辰生物科技
有限公司